Introduction
Purposes:
1) Can be used for multiple types of large scale DNA quantification including, a fluorometric based approach using a picoGreen assay (reagents needed) or an absorbance based approach with the Take3 insert (similar to a nanodrop; no reagents needed).
Location: Shared Molecular Lab
Protocols:
- Picogreen dsDNA Assay Quantification Assay Protocol This protocol is for performing the equivalent of “qubit” (fluorometric) on a whole plate. It’s always good to cross-check a few of these outputs against quibit measures.
Reagents and Supplies:
- PicoGreen Kits - Link - Sold by multiple vendors.